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Scientific 
Experience

BIT510 Core Technologies in Molecular and Cellular Biology 
Explored basic techniques in DNA cloning, protein expression in prokaryotic and eukaryotic systems, and protein purification. 

Skills Gained 
 

  • DNA Purification
  • PCR Amplification
  • Gel Electrophoresis
  • Gibson Assembly Cloning
  • Screening via Colony PCR
  • Screening via Sanger Sequencing
  • Western Blot
  • ImageJ Analysis
  • Bradford Assay
  • Mammalian Cell Culture Passaging
  • Mammalian Cell Transfection


 
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UV light image of e.coli pET-41a(+)-CaMPARI on LB + Carb

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UV light image of flow-through, wash, and elution fractions collected during glutathione affinity chromatography of GST::CaMPARI.

Quality Laboratory Specialist 
Creature Comforts Brewing Co.
                 Athens, GA                     
July 2023 - July 2024

●    Conducted thorough quality control tests on raw materials and finished products, ensuring compliance with industry standards and regulatory requirements.
●    Developed and implemented standardized testing procedures, improving efficiency and consistency in laboratory operations.
●    Maintained accurate records of laboratory activities, including test results and quality assurance reports, ensuring traceability and compliance with ISO 9001 standards. 
●    Utilized statistical analysis software to analyze data trends, identify areas for improvement, and support quality assurance initiatives. 

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Using microscopy to identify microbial contaminants 

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Filter plating brite tank samples on different selective medias

Skills Gained 
 

  •  GC-MS characterization of  volatile compounds
  • Nexcelom Cellometer Cell Counting
  • Hemocytomer Cell Counting 
  • Filter plating
  • Streak plating
  • Flood plating 
  • Making medias
  • qPCR identification of microbial contaminants
  • Microscopy identification of microbial contaminants
  • Data management in PowerBI  


 
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Undergraduate Research Laboratory Assistant 
        University of Georgia | Athens, GA   
  January 2022 - August 2023

●    Assisted in the design and implementation of experiments to characterize enzyme kinetics, contributing to the understanding of substrate interactions and reaction mechanisms.
●    Conducted purification and analysis of enzymes using techniques such as chromatography and electrophoresis to achieve high purity levels and yield.
●    Utilized spectrophotometry and other analytical techniques to quantify enzyme activity, generating data for publication and presentations.
●    Maintained detailed lab records and documentation of experimental protocols, results, and observations to ensure reproducibility and compliance with research standards.

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Skills Gained 
 

  • Plasmid transformation 
  • Plasmid Cloning
  • Cell culture
  • Protein purification 
  • Fast Protein Liquid Chromatography (FPLC)
  • High Performance Liquid Chromatography (HPLC)
  • Protein crystallization
  • Iron Reconstitution
  • Anaerobic Glovebox Work 
  • Liquid Phase Oxygen Electrode
  • UV-Vis Spectrometry
  • Hanging Drop Vapor Diffusion Crystallization


 
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Learn more about the Wang lab's characterization of RufO 

RufO Structure

Learn more about my work characterizing a non-heme thiol dioxygenase - Mercaptosuccinate Dioxygenase

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MSDO Reaction Scheme
 

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